Chimie Bioorganique et Bioinorganique
1.4. Cancer biomarkers AMF/PGI
(collab. Theme 1 & Theme 3)
Objective:
Design of an electrochemical biosensor for the early diagnosis of cancers.
Strategy:
Detection of a validated tumor biomarker, autocrine motility factor (AMF): cytokine potentially involved in the development of metastases in vivo - AMF (extracellular protein) = PGI (phosphoglucose isomerase, intracellular glycolitic enzyme).
Résults:
Elaboration of a biosensor based on the bioreceptor substrate of PGI, D-fructose-6-phosphate (F6P):
- Limit of quantification : 10 fM
- Dynamique range: 10 fM to 1 pM
- Biosens. Bioelectron. 2017, 96, 178-185
